Rolipram treatment alone and in combination with BDNF improved the survival of SGN.

<p>The neuronal survival was assessed by the amount of surviving neurons after a cultivation period of 48(% neuronal survival). Rolipram (R) applied at a concentration of 0.1 nM enhanced the survival of SGN after serum deprivation and resulted in a significant higher survival rate when compared to the untreated group and to the positive control (treated with BDNF [50 ng/ml]). Elevated Rolipram concentrations (1, 3, 10 nM) did not result in an increased neuronal survival compared to the untreated control group. Co-treatment with Rolipram and BDNF caused an additional increase of neuronal survival independently of varying Rolipram concentrations. Values are given as mean ± SEM; N = 4; n = 3; One-way ANOVA with Tukey's multiple post hoc test was used to compare means: *p<0.05; **p<0.01; ***p<0.001. Reference of the significance is marked by the thick bar. N quotes the number of independent experiments; n gives the number of repetitions of each condition within one experiment.