Frequencies of Tim-3 and PD-1 expressing T cells compared over the course of WNV infection in groups with different disease outcome.

<p>The graphs below demonstrate the change of frequencies of (A) Tim-3⁺, (B) PD-1⁺, (C) Tim-3⁺PD-1⁻, and (D) Tim-3⁺PD-1⁺ CD4⁺ (left panel) and CD8⁺ (right panel) T cell subsets in asymptomatic (AS, circles/solid lines, n = 24) and symptomatic (S, squares/dash lines, n = 8) WNV-infected subjects 14, 30, 90, and 365 days post-index donation. The frequencies of the same cells in uninfected controls are displayed (WNV−, triangles, n = 26). The symbols indicate the means and the error bars represent the SEM. The <i>p</i>-values of the pairwise comparisons between asymptomatic and symptomatic groups are indicated by *<i>p</i> <0.05, ** <i>p</i> <0.01, and *** <i>p</i> <0.001 above time-points when groups were compared at a given time-point by Mann-Whitney test. The <i>p</i>-values of the comparison between asymptomatic and symptomatic groups are indicated by ** adjacent to the cell subset title for <i>p</i> <0.01 over the time of post-index by generalized estimated equation (GEE).