<p>Vaccine induced T cell responses detected in ELISPOT above the threshold of 50 IFN-γ spot forming cells (SFCs) at two weeks prior to SIV infection were tracked for the first six weeks post infection and are shown for animals [<b>A</b>] r07015, [<b>B</b>] r99047, and [<b>C</b>] r99080 from vaccine group one and [<b>D</b>] rh1999, [<b>E</b>] r05040, and [<b>F</b>] r99079 from vaccine group two. Similar results were obtained for the remaining animals in both groups. The results shown indicate the mean plus standard deviation of duplicate wells for the indicated peptide pools with the background level subtracted. Time from last ERE vaccination [Ad5 for group 1, DNA for group 2] is indicated at the top of each graph.</p
<p>We determined the breadth of T-cell responses in each animal by adding the number of positive IFN...
Wild-type C57BL/6 or IFN-γ.eYFP reporter mice were either naïve or i.v. injected with 200cfu BRD509 ...
<p><b>A</b> & <b>B</b>) Balb/c mice were immunized i.m. at weeks 0 and 2 with 50 µg of pSG2-Ag85A or...
<p>We used the same approach described in the legend of <a href="http://www.plosone.org/article/info...
<p>We used IFN-γ ELISPOT to determine the magnitude of vaccine-induced T-cell responses at weeks 1, ...
<p>A) IFN-<b>γ</b> analysis was performed on peripheral blood mononuclear cells. Vaccine-induced res...
<p>Groups of 4 BALB/c mice were vaccinated with 10<sup>10</sup> vp/mouse of either PAV3-HA () or AdH...
<p>Mice were immunized intragastrically at day 0, 14, and 28 with PBS, the commercial H5N1 vaccine (...
<p>We detected T cell responses in peripheral blood mononuclear cells (PBMC) using an IFN-γ elispot ...
<p><b>A)</b> PBMCs isolated from blood samples taken at the indicated weeks post-immunization (PI) w...
<p>(<b>A</b>) The magnitude of the SIV-specific IFN-γ T cell response in the gut was determined by E...
<p><sup>a</sup> Number of IFNγ secreting cells/10<sup>6</sup> splenocytes were removed at the indica...
<p>Groups of mice (n = 6/group) were primed i.d. with Ad5, MVA or Ad5+M expressing Ag85A and receive...
<p>SIV Gag-specific CD4<sup>+</sup> T cell responses were measured by intracellular cytokine stainin...
<div><p>(A) IFN-γ secreting T cell frequencies in PBMC from protected animals (D68–D14) compared to ...
<p>We determined the breadth of T-cell responses in each animal by adding the number of positive IFN...
Wild-type C57BL/6 or IFN-γ.eYFP reporter mice were either naïve or i.v. injected with 200cfu BRD509 ...
<p><b>A</b> & <b>B</b>) Balb/c mice were immunized i.m. at weeks 0 and 2 with 50 µg of pSG2-Ag85A or...
<p>We used the same approach described in the legend of <a href="http://www.plosone.org/article/info...
<p>We used IFN-γ ELISPOT to determine the magnitude of vaccine-induced T-cell responses at weeks 1, ...
<p>A) IFN-<b>γ</b> analysis was performed on peripheral blood mononuclear cells. Vaccine-induced res...
<p>Groups of 4 BALB/c mice were vaccinated with 10<sup>10</sup> vp/mouse of either PAV3-HA () or AdH...
<p>Mice were immunized intragastrically at day 0, 14, and 28 with PBS, the commercial H5N1 vaccine (...
<p>We detected T cell responses in peripheral blood mononuclear cells (PBMC) using an IFN-γ elispot ...
<p><b>A)</b> PBMCs isolated from blood samples taken at the indicated weeks post-immunization (PI) w...
<p>(<b>A</b>) The magnitude of the SIV-specific IFN-γ T cell response in the gut was determined by E...
<p><sup>a</sup> Number of IFNγ secreting cells/10<sup>6</sup> splenocytes were removed at the indica...
<p>Groups of mice (n = 6/group) were primed i.d. with Ad5, MVA or Ad5+M expressing Ag85A and receive...
<p>SIV Gag-specific CD4<sup>+</sup> T cell responses were measured by intracellular cytokine stainin...
<div><p>(A) IFN-γ secreting T cell frequencies in PBMC from protected animals (D68–D14) compared to ...
<p>We determined the breadth of T-cell responses in each animal by adding the number of positive IFN...
Wild-type C57BL/6 or IFN-γ.eYFP reporter mice were either naïve or i.v. injected with 200cfu BRD509 ...
<p><b>A</b> & <b>B</b>) Balb/c mice were immunized i.m. at weeks 0 and 2 with 50 µg of pSG2-Ag85A or...