Measurement of vaccine induced immunogenicity one week after each vaccination.

<p>Groups of mice (n = 6/group) were primed i.d. with Ad5, MVA or Ad5+M expressing Ag85A and received a homologous boost at 8 weeks and 16 weeks after the prime (A). IFN-γ release by PBMC stimulated with the CD4+ epitope p15 (B) and CD8+ epitope p11 (C) was measured 1 week after each vaccination by ELISpot. The frequency of CD4+ IFN-γ+ (D) and CD8+IFN-γ+ splenocytes (E) 1 week after the second boost was evaluated by intracellular staining. Data is representative on one experiment. The median and interquartile range is displayed (B–E).