Characteristics of sequencing GC bias.

<p>A, Coefficient of Variation of sequencing reads from two platforms on each chromosome. This correlation between CVs of UR% from 18 samples (y-axis) and GC-content of each chromosome (x-axis, sorted by GC content) are shown in this figure. The color-code lines represented data generated on MiSeq (blue) and Ion Proton (red) platform, respectively. B, Average RD in each GC content category. The blue line (MiSeq) and red line (Ion Proton) shows the relative depth (RD, y-axis) at each sequencing GC-content 1 Mbp windows (x-axis). The dash lines represent the RD from 0.80 to 1.20.