Evaluation of the detection of low-percentage variants on human blood DNA by using (A) DHPLC (B) and SN/WAVE-HS.

<p>The SURVEYOR Plus Mutation Kit was used for SN/WAVE-HS. Templates with different percentages of the DNA from blood B (designated as bld. B) were prepared by mixing the DNA from blood B with the DNA from blood A. The front heteroduplex peaks detected by DHPLC are indicated by *. ▾ indicates the expected 39-bp and 190-bp DNA fragments after SN digestion. The numbers above the peaks of the DNA ladder indicated the sizes of the DNA markers.