Results from SN/WAVE-HS and DHPLC analysis of the DM#38 amplicon by using total DNA and mtDNA from cells.

<p>(A) Results from SN/WAVE-HS analysis. (B) Results from DHPLC analysis. The upper and lower panels of Figure 6A and Figure 6B display the results for 143B cells and the cybrid cells, respectively. In Figure 6A, the results for the DNA samples without (uncut) and with (cut) SN digestion were also compared. ▾ indicates the peaks that appeared in the chromatograms of uncut DNA but were not observed in the chromatograms of the corresponding cut DNA for all DNA samples. ▽ indicates the peaks >100 bp only present in the chromatograms of the uncut DNA of both total DNA and mtDNA (m), but not the cut DNA. Such peaks for the mtDNA were considerably smaller than those of total DNA. ↓ indicates the peaks <100 bp that were present in the chromatograms of the uncut total DNA but absent in those of the uncut mtDNA. The patterns of peaks in the chromatograms from uncut DNA and cut DNA displayed marked differences. The chromatograms of the cut DNA samples displayed numerous small peaks. One obvious peak (indicated by *) appeared for the cut DNA of whole cells but was absent for mtDNA. In Figure 6B, the small but identifiable heteroduplex peaks detected by DHPLC are indicated by #.