HBx does not reduce miR-122 in hepatic cells via transcriptional regulation.

<p>(A) qRT-PCR assay for miR-122 in HBs-, HBc-, HBp-, or HBx-expressing cells. (B) qRT-PCR analysis for miR-122 in HBx-silenced HepG2.2.15. (C) qRT-PCR analysis for miR-122 in three different cell lines (QSG7701, HepG2, Huh7) transfected with pHBx at different doses and the corresponding controls. (D) Dual-luciferase assay of the miR-122 promoter in QSG7701, HepG2, and Huh7 cells transfected with pHBx and controls. (E) qRT-PCR analysis for pre-miR-122 in HepG2 and HepG2.2.15 cells. (F) qRT-PCR analysis for pre-miR-122 in three different cell lines (QSG7701, HepG2, Huh7) transfected with pHBx at different doses and the corresponding controls. The data represent the mean ± SD. (*p<0.05, **p<0.01, ***p<0.001).