Effect of HBx on PDCD4 and PTEN.

<p>HBx protein was over-expressed in both Huh 7 (A) and Hep G2 (B) cells and the expression of miRNA-21 was estimated using real time PCR (n = 3; *p<0.001). HBx was over-expressed in both Huh 7 and Hep G2 cells and the target proteins for miRNA-21 were determined using Western blots. This picture is a representative of the three experiments (C). Lane 1, Control; lane 2, Empty vector; and lane 3, HBx transfected cells. β-actin was used as loading control. These Western blots were quantified using Li-COR's image studio lite software and the data are presented for Huh 7 (D) and Hep G2 (E) cells (n = 3; *p<0.05).