Protection of DNA against Dnase I digestion using gels stained with GelStar.

<p>(A) G4/DNA complexes with <i>r</i>_charge = 0.4 are used and the untreated complex is loaded on lane 1. The 2^nd lane displays the dissociated complex after treatment with 10 μg mL⁻¹ heparin for 30 min. All other samples (lanes 3–7) are treated with 1 unit of Dnase I for the indicated time periods. To the samples in lanes 4–7, heparin was added after the Dnase I enzyme was heat inactivated. (B) Linearized plasmid DNA only is loaded onto lane 1 and the sample loaded onto lane 2 contains DNA, treated with Dnase I for 30 min. Samples loaded onto lanes 3–7 contain DNA and CTAB (<i>r</i>_charge = 7.5). Samples loaded onto lanes 4–7 are treated with Dnase I for the time periods indicated.