Protection of DNA from Dnase I digestion.

<p>(<b>a</b>) Sso10b and its triple mutant. and (<b>b</b>) Mth10b and its triple mutant: Lane 1, plasmid alone; lane 2, 1% SDS alone; lane 3, plasmid with addition of SDS; lane 4, plasmid digested with 0.5 U Dnase I before addition of SDS; lane 5, triple mutant incubated with 0.5 U Dnase I before addition of SDS; lane 6, DNA incubated with the triple mutant then digested with 0.5 U Dnase I before addition of SDS; lane 7, wild type incubated with 0.5 U Dnase I before addition of SDS; lane 8, DNA incubated with wild type then digested with 0.5 U Dnase I before addition of SDS. (<b>c</b>) Mth10b, Sso10b and their single mutants: Lane 1, plasmid alone; lane 2, plasmid with addition of SDS; lane 3 to 10, DNA incubated with Mth10b, N11K, I39R, T77R, Sso10b, K16N, R44I and R83T then digested with 0.01 U Dnase I before addition of SDS.