Antenna-less females mutant for <i>ppk25, ppk23 or ppk29</i> are unreceptive to males.

<p>A. The percentage of females that mated within 30(“Receptivity”) was calculated for females with homozygous null mutations in <i>ppk25</i>, <i>ppk23</i> or <i>ppk29 </i><a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004238#pgen.1004238-Liu1" target="_blank">[11]</a>, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004238#pgen.1004238-Thistle1" target="_blank">[13]</a>, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004238#pgen.1004238-Lin1" target="_blank">[16]</a>, and for control females with one normal copy of <i>ppk25</i>, <i>ppk23</i> and <i>ppk29</i>. N = 30–34. For receptivity in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004238#pgen-1004238-g005" target="_blank">Fig. 5</a> and <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004238#pgen-1004238-g006" target="_blank">6</a>, error bars indicate the SEM. B. Antenna-less females mutant for <i>ppk25</i>, <i>ppk23 or ppk29</i> show reduced receptivity to males. Receptivity was measured for mutants and control females as in A but after removal of antennae. N = 30–38; ***p<0.001. (Fisher's exact test). C. Arista-less females mutant for <i>ppk23 or ppk25</i> show reduced receptivity to males. Receptivity was measured for mutants and control females as in A but after removal of arista. N = 21–32; ***p<0.001. (Fisher's exact test). D. <i>wt</i> males find <i>ppk25</i> mutant and control females equally attractive. CI measured for Canton-S males toward females that were either alive (left) or decapitated (right). CI of males toward live females was quantified by measuring male courtship behavior during the first 10 minutes of the receptivity assay shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004238#pgen-1004238-g005" target="_blank">Fig. 5B</a>. Females were either homozygous null mutants for <i>ppk25</i> or contained one normal copy of <i>ppk25</i>. N = 20. E. Introduction of a <i>ppk25</i> transgene increases receptivity of <i>ppk25</i> mutants but not of <i>ppk29</i> mutants. Receptivity was measured for antenna-less females carrying an <i>UAS-ppk25</i> transgene in either a <i>ppk25</i> mutant or <i>ppk29</i> mutant background and compared to <i>ppk25</i> mutant and controls shown earlier (panel A). N = 30–38; ***p<0.001 (Fisher's exact test).