Results of pair mating and locomotor experiments using <i>dati¹</i> and UAS-<i>dati</i>-RNAi.

<p>(A, C, E) Percentage of females that accepted wild-type Canton-S male courtship within 1 h in pair mating assays. (B, D, F) Courtship indices of Canton-S males towards females tested in (A), (C), and (E), respectively. Courtship indices were arcsin transformed and subject to one-way ANOVA, and in all cases they are not significantly different from each other (<i>p</i>>.05). Error bars represent ±SEM of the measurements of each genotype. (A–F) Genotypes of females tested are indicated (“UAS-RNAi” means UAS-<i>dati</i>-RNAi). Dark and light bars in (C–F) indicate experimentals and controls, respectively. (A, B) <i>dati</i>¹ homozygous females strongly reject males, despite being courted vigorously by Canton-S males. This rejection phenotype is reverted in revertant females homozygous for the precise excision <i>dati^F11.4</i> allele. Controls with Canton-S and <i>y w</i> females were used, as revertants lose both <i>y</i>+ and <i>w</i>+ transgenes. (C, D) Ubiquitous expression of UAS-<i>dati</i>-RNAi driven by <i>Act</i>-Gal4 or expression in postmitotic neurons driven by <i>elav</i>-Gal4 causes rejection of male courtship. (C) Dark bars indicate the percentage of females expressing UAS-<i>dati</i>-RNAi driven by different Gal4 drivers that accepted male courtship. Light bars indicate results with control females (Canton-S females and females with either UAS-<i>dati</i>-RNAi or a Gal4 driver). Expression of <i>dati</i>-RNAi in glial cells (<i>repo</i>-Gal4) does not cause rejection. (E, F) Expression of <i>dati</i>-RNAi specifically in cholinergic neurons causes the female rejection behavior. (E) Percentage of courtship acceptance in females expressing UAS-<i>dati</i>-RNAi driven by neuron-specific Gal4 drivers (dark bars) versus control females with either Gal4 drivers or UAS-<i>dati</i>-RNAi only (light bars). <i>Ddc</i>-Gal4 is a driver of both serotonergic and dopaminergic neurons, <i>ple</i>-Gal4 of dopaminergic neurons, <i>Gad</i>-Gal4 of GABAergic neurons, and <i>Cha</i>-Gal4 of cholinergic neurons. (G) Courtship acceptance and locomotor deficits in <i>dati</i> mutant females are separable phenotypes. The graph shows the distance climbed by females of various genotypes within 5 s after being knocked to the bottom of a vial. Measurements shown are an average of five replicates from each group; error bars represent the mean ± the standard deviation. Sample size numbers are indicated inside bars in (A, D, E, G). Graphs (B, D, F) use the same datasets as (A, C, E), respectively. Statistical significance of differences in (A, C, E, G) was evaluated by the Fisher's exact probability test (***<i>p</i><0.001; **<i>p</i><0.01; *<i>p</i><0.05).