Effect of 5-azaC on the apoptosis and cell cycle in NPC cells <i>in vitro</i>.

<p>(A) CNE2 and SUNE1 cells were treated with 1 μmol/L 5-azaC and/or irradiated (IR) at 6 Gy. The rate of apoptosis (Annexin V (+)/PI (−) plus Annexin V (+)/PI (+)) was measured by flow cytometry at 72 hours after treatment (Student’s <i>t</i>-test, **<i>P</i><0.01; ***<i>P</i><0.001). (B) CNE2 and SUNE1 cells were treated with 5-azaC or PBS for 24 h and stained with PI to examine the cell cycle distribution by flow cytometry (Student’s <i>t</i>-test, <i>p</i>>0.05). Representative flow cytometric plots and quantification of the cell cycle distribution are shown.