Effect of 5-azaC on the DNA methylation and expression of representative tumor suppressor genes that are hypermethylated and silenced in NPC.

<p>SUNE1 and CNE2 cells and mice bearing CNE2 tumor xenografts were treated with or without 5-azaC. (A) DNA methylation pyrograms for <i>RASSF1A</i> and <i>RPRM</i> in CNE2 cells. (B) Mean levels of DNA methylation for <i>RASSF1A, RPRM, CDKN2A</i> and <i>14-3-3σ</i> (Student’s <i>t</i>-test, *<i>p</i><0.05, **<i>p</i><0.01). (C) Real-time PCR analyses of <i>RASSF1A, RPRM, CDKN2A</i> and <i>14-3-3σ</i> (Student’s <i>t</i>-test, **<i>P</i><0.01).