IGF2 infusion increases BMP9 expression and modulates the levels of its receptor, ALK1.

<p>Septal lysates from WT/CHGFP and APP.PS1/CHGFP mice were analyzed by RT-PCR to determine <i>Bmp9</i> mRNA levels (A,B). Hippocampal lysates were used to determine BMP9 and ALK1 protein levels by immunoblot (C,D,E). Data were analyzed by two-way ANOVA followed by a post-hoc Fisher’s LSD test. IGF2 infusion increased the expression of BMP9 mRNA levels within the septum [F(1, 20) = 12.885, p = 0.002]. Significant differences in BMP9 mRNA between groups are indicated by * (p = 0.003) (B, see bracket). BMP9 protein levels in the hippocampus were also significantly increased by the infusion of IGF2 [F(1, 20) = 21.770, p = 0.0002]. Significant differences in BMP9 protein level between groups are indicated by * (p = 0.001), # (p = 0.025), and † (p = 0.046) (D, see brackets). IGF2 treatment decreased the expression of ALK1 protein [F(1, 20) = 5.724, p = 0.026]. Significant differences in ALK1 protein level between groups are indicated by * (p = 0.031) (E, see bracket).