ADAMTS-1 expression is reduced in LV-shRNA-ADAMTS-1 transduced vessels.

<p><b>A</b>) is representative sections using confocal microscopy from the outflow vein after transduction with LV-shRNA-ADAMTS-1 (<b>LV</b>) and control shRNA (<b>C</b>) at day 7 (<b>D7</b>) and 14 (<b>D14</b>). Both the <b>C</b> and <b>LV</b> have a GFP tag. First column shows DAPI intensity indicated by blue staining nuclei. Second column shows GRP intensity. Magenta staining cells are GFP positive. Third column depicts merged images. These sections demonstrate that there is <b>LV</b> or <b>C</b> delivery to the adventitia and endothelium of the vessel wall. By day 14, the gfp signal decreases and is not detectible. <b>B</b>) is the pooled data of the mean gene expression of ADAMTS-1 at the outflow vein after transduction with LV-shRNA-ADAMTS-1 (<b>LV</b>) compared to control shRNA (<b>C</b>) using qPCR analysis at day 7 (<b>D7</b>), 14 (<b>D14</b>), and 21 (<b>D21</b>). This demonstrates a significant reduction in the mean ADAMTS-1 expression in the <b>LV</b> transduced vessels when compared to <b>C</b> vessels at <b>D7</b> (P<0.0001). <b>C</b>) is representative sections from ADAMTS-1 staining at the outflow vein of <b>LV</b> transduced vessels when compared to <b>C</b> vessels at day 14. Cells staining brown are positive for ADAMTS-1. IgG antibody staining was performed to serve as negative control. <b>D</b>) is the pooled data of the mean ADAMTS-1 staining at the outflow vein after transduction with <b>LV</b> compared to <b>C</b> at day 7 (<b>D7</b>), 14 (<b>D14</b>), and 21 (<b>D21</b>). This shows that by <b>D14</b>, there is a significant reduction in the mean ADAMTS-1 staining in the <b>LV</b> transduced vessels when compared to <b>C</b> (P<0.0001). All are 40X. Scale bar is 50-μM. * Indicates the lumen. Each bar shows mean ± SEM of 4-6 animals per group (<b>B, D</b>). Two-way ANOVA followed by Student <i>t</i>-test with post hoc Bonferroni's correction was performed. Significant difference from control value was indicated by ^##<i>P</i><0.0001.