<i>Xcc</i>MAFF106712 is virulent for Arabidopsis ecotypes Col-0 plants in a type III dependent manner.

<p>(A) In planta bacterial growth of <i>Xcc</i>MAFF106712 after inoculation on Arabidopsis ecotypes Col-0 (solid) and Sf-2 (gray). Twenty-four leaves from 8 different plants were inoculated by piercing the leaves with an <i>Xcc</i>MAFF106712 suspension of 10⁹ cfu mL⁻¹. Enhanced bacterial multiplication in Col-0 compared to Sf-2 was significant (*P<0.001, Student’s t test). Results are representative of at least three experiments per condition. (B) <i>Xcc</i> MAFF106712 pathogenicity on Col-0 is dependent on the type III secretion system. Bacteria (<i>Xcc</i>MAFF106712 and MAFF106712Δ<i>hrcC</i>) were inoculated on Col-0 and Sf-2 plants and photographs were taken 15 days post inoculation. Black rot phenotype of Col-0 plant 7 days post <i>Xcc</i>MAFF106712 inoculation is shown in the window. At 7 dpi, some infected leaves are still green and some of the leaves start showing the disease phenotype. We used mixed up them for the growth assay shown in the panel A. Red arrows indicate necrotic leaves caused by bacteria inoculation.