Validation of crosslinking and immunoprecipitation of RLR/RNA complexes from 24 h virus-infected cells.

<p><b>A:</b> Schematic representation of the experimental procedure for characterization of RLR-associated RNA molecules. <b>B:</b> Immunostimulatory activity of RNA from RIG-I and control (GFP) crosslinking samples in comparison to non-crosslinking immunoprecipitates. <b>C:</b> Western blot analysis of crosslinked and non-crosslinked RIG-I and control (GFP) pull-down experiments. <b>D:</b> Comparison of RNA recovery levels by quantitative PCR analysis of RIG-I-associated RNA from SeV-infected cells (n = 3). n.d. = not detectable.