Western blot profiles.

<p><i>H. pylori</i> ATCC 43504 urease was treated with dehydrocostus lactone (DCL) and (–)-epigallocatechin gallate (EGCG). The UreA (monomer) and UreB (dimer) bands were confirmed by Western blot with antibodies toward <i>H. pylori</i> urease. The UreB band and UreA dimer and monomer bands were not disappeared at 10 mM DCL. However, the UreA monomer band completely disappeared at 0.1 mM EGCG, and the UreA dimer and UreB bands were faint at the same concentration of the compound. Lanes: 1, ATCC 43504 urease; 2, 2 mM DCL; 3, 5 mM DCL; 4, 10 mM DCL; 5, 0.1 mM EGCG; 6, 0.2 mM EGCG; 7, 0.5 mM EGCG.