SDS-PAGE electrophorograms of native <i>H</i>. <i>pylori</i> J99 urease partially purified from the culture supernatant.

<p>Cells were grown for 72 h in the presence of 15 mM urea. M–molecular weight marker, SE–a soluble protein fraction before purification, PhS–fractions containing active urease after hydrophobic interaction purification using Phenyl Sepharose, CM–original culture medium (control). The red arrows indicate α and β subunits of urease with molecular weights of 61.7 and 29.5 kDa, respectively.