AKAP12 KO mice showed larger demyelinated and infarcted tissue compared to WT from the extended infiltration of immune cells.

<p>(A) The areas infiltrated by CD45-positive leukocytes increased in the AKAP12 KO mice on day 21 after injury. Brain sections were co-stained with antibodies against fibronectin and CD45 (the marker for pan leukocytes). Scale bar: 500 µm (left panel), 200 µm (magnified panel) (FS: fibrotic scar) (B) The AKAP12 KO mice showed more severe inflammation compared to the WT mice. The areas infiltrated by the IBA1-positive immune cells and the number of IBA1-positive cells per unit area near the lesion site increased in the AKAP12 KO mice on day 21 after injury (mean ± S.D.; n = 6 mice per WT and KO and 3 positions per mouse; P#<0.001). Scale bar: 200 µm (C) The AKAP12 KO mice showed increased demyelination compared to the WT. Luxol fast blue (LFB) staining was performed to evaluate demyelination (the asterisk region) in the injured brain (mean ± S.D.; n = 4 mice per WT and KO; P**<0.01). Scale bar: 2 mm (D) The AKAP12 KO mice showed increased tissue damage compared to the WT. Triphenyltetrazolium chloride (TTC) staining to evaluate the infarct volume (Mean ± S.D.; n = 4 mice per WT and KO; P#<0.001). Scale bar: 2 mm.