γHV-68 EAE mice show MS-like lesions in the white matter of the cerebellum and in the corpus callosum.

<p>Mice were infected with γHV-68 or MEM only. Five weeks p.i. EAE was induced. At day 28 post EAE induction mice were perfused and brains were embedded in OCT, snap frozen, cut and stained with antibodies specific for CD4, CD8 and F4/80; or were formalin fixed and embedded in paraffin and stained with luxol fast blue. (<b>A–B</b>) The images displayed for CD4-CD8-F4/80 staining are consecutive sections cut from the same cerebral hemisphere of the same mouse (all images from γHV-68 EAE mice). Scale bar: 50 µm. (<b>C</b>) The arrows show an area of demyelination in the cerebellum of a γHV-68 EAE mouse and the asterisks highlight immune cell infiltrations. The right panel shows a normal cerebellum from a naïve EAE mouse. Scale bar: 100 µm. All pictures are representative of two separate experiments (n = 16/group).