<p>A. <i>left</i> - EGFP fluorescence in living photoreceptor cells expressing EGFP-PDE6β1-313, <i>center</i> - DIC image, <i>right</i> - EGFP-fluorescence/DIC overlay. <i>Bar</i> - 10 µm. B. Co-localization of EGFP-PDE6β1-313 (green) with the rod outer segment marker Wheat Germ Agglutinin (red) in cryosections of transgenic retina. Cryosections were counterstained with TO-PRO3 nuclear stain (blue). <i>Bar</i> - 10 µm. C. Isotonic (I), hypotonic (H) extracts of transgenic EGFP-PDE6β1-313 retinas were analyzed by immunoblotting with anti-GFP B-2 monoclonal antibody.</p
AbstractPrevious studies by Papermaster and coworkers introduced the use of rhodopsin–green fluoresc...
<p><i>Shaker1</i> mouse retinas were co-transduced by a subretinal injection of UshStat and EIAV-CMV...
<p>(<b>A–D</b>) Expression of EGFP in the eye of a single adult <i>Tg(krt5:Gal4-ERT-VP16; UAS:EGFP)<...
<p>A. <i>left</i> - EGFP fluorescence in living photoreceptor cells expressing PDE6β1-314fs*50, <i>c...
<p>Adult transgenic frogs expressing Rho-eGFP, which has been modified to contain an epitope for the...
Sublayer distributions of virus-transduced enhanced green fluorescent protein (EGFP)-positive cells ...
Enhanced green fluorescent protein (EGFP; ), proliferating cell nuclear antigen (PCNA; ), Pax6 (), g...
<p>(A–E) A retinal explant from transgenic frogs expressing soluble arrestin-eGFP in rods was dark a...
Enhanced green fluorescent protein (EGFP; ), proliferating cell nuclear antigen (PCNA; ), and merged...
<p>(A) At 42 hpf, EGFP is detected throughout the retinal neuroepithelium in the central retina. PCN...
<p>(A) At 48 hours after light onset, almost all rod photoreceptors have been ablated and proliferat...
<p>Sections were immunolabeled with EGFP (green) to visualize the <i>nrd:egfp</i> transgene and Zpr-...
<p>The main images a–d are confocal stacks illustrating overall GFP expression patterns. For each se...
<p>(A). Schematic representation of EdU injections during the light time course with corresponding i...
<p>(A) Schematized generation of the <i>r-opsin1::egfp-f2a-ntr</i> construct. The <i>egfp-f2a-ntr</i...
AbstractPrevious studies by Papermaster and coworkers introduced the use of rhodopsin–green fluoresc...
<p><i>Shaker1</i> mouse retinas were co-transduced by a subretinal injection of UshStat and EIAV-CMV...
<p>(<b>A–D</b>) Expression of EGFP in the eye of a single adult <i>Tg(krt5:Gal4-ERT-VP16; UAS:EGFP)<...
<p>A. <i>left</i> - EGFP fluorescence in living photoreceptor cells expressing PDE6β1-314fs*50, <i>c...
<p>Adult transgenic frogs expressing Rho-eGFP, which has been modified to contain an epitope for the...
Sublayer distributions of virus-transduced enhanced green fluorescent protein (EGFP)-positive cells ...
Enhanced green fluorescent protein (EGFP; ), proliferating cell nuclear antigen (PCNA; ), Pax6 (), g...
<p>(A–E) A retinal explant from transgenic frogs expressing soluble arrestin-eGFP in rods was dark a...
Enhanced green fluorescent protein (EGFP; ), proliferating cell nuclear antigen (PCNA; ), and merged...
<p>(A) At 42 hpf, EGFP is detected throughout the retinal neuroepithelium in the central retina. PCN...
<p>(A) At 48 hours after light onset, almost all rod photoreceptors have been ablated and proliferat...
<p>Sections were immunolabeled with EGFP (green) to visualize the <i>nrd:egfp</i> transgene and Zpr-...
<p>The main images a–d are confocal stacks illustrating overall GFP expression patterns. For each se...
<p>(A). Schematic representation of EdU injections during the light time course with corresponding i...
<p>(A) Schematized generation of the <i>r-opsin1::egfp-f2a-ntr</i> construct. The <i>egfp-f2a-ntr</i...
AbstractPrevious studies by Papermaster and coworkers introduced the use of rhodopsin–green fluoresc...
<p><i>Shaker1</i> mouse retinas were co-transduced by a subretinal injection of UshStat and EIAV-CMV...
<p>(<b>A–D</b>) Expression of EGFP in the eye of a single adult <i>Tg(krt5:Gal4-ERT-VP16; UAS:EGFP)<...