Expression of PDE6β1-314fs*50 in transgenic <i>X. laevis</i> rods photoreceptors.
- Gaël Manes (554905)
- Pallavi Cheguru (554906)
- Anurima Majumder (554907)
- Béatrice Bocquet (554908)
- Audrey Sénéchal (554909)
- Nikolai O. Artemyev (266810)
- Christian P. Hamel (298436)
- Philippe Brabet (298437)
Publication date
April 2014
DOIAbstract
<p>A. <i>left</i> - EGFP fluorescence in living photoreceptor cells expressing PDE6β1-314fs*50, <i>center</i> - DIC image, <i>right</i> - EGFP-fluorescence/DIC overlay. <i>Bar</i> - 10 µm. B. Co-localization of PDE6β1-314fs*50 (green) with the rod outer segment marker Wheat Germ Agglutinin (red) in cryosections of transgenic retina. Cryosections were counterstained with TO-PRO3 nuclear stain (blue). <i>Bar</i> - 10 µm. C. Isotonic (I), hypotonic (H) extracts of transgenic PDE6β1-314fs*50 retinas were analyzed by immunoblotting with anti-GFP B-2 monoclonal antibody.</p
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