Contribution of antioxidant systems on contractile responses to noradrenaline in both mesenteric arteries and the mesenteric bed.

<p>Effect of HFD on ec-SOD protein expression in mesenteric PVAT [A] and in MA [B]. Diagram bars show the result of densitometric analysis of ec-SOD immunoblots, expressed as percentage of ec-SOD in the control (C) group. Data are means ± S.E.M. (n≥5 animals per group). Total SOD activity in mesenteric PVAT [C] and in MA [D]. Data are presented as means ± S.E.M. (n≥5 animals per group). *p<0.05 compared to C animals. [E] Cumulative concentration-response curves to noradrenaline (NA, 0.1–10 µM) in MB and [F] cumulative concentration-response curves to NA (0.1–10 µM) in MA from C and HFD animals in absence/presence of 3-amino-1,2,4-triazole (3-AT, 20 mM). Data are means ± S.E.M. (n≥5 animals per group). ^##p<0.01; ^###p<0.001 compared to their corresponding matched control groups.