Temperature-dependent changes of the <i>M₁</i> first moment versus temperature, for different samples as shown in each case.

<p>(A) POPC/POPC-d₃₁ (2∶1, molar ratio); POPC/POPC-d₃₁ (2∶1, molar ratio) in the presence of C2α domain (40∶1 phospholipid/protein molar ratio); POPS/POPS d₃₁ (2∶1 molar ratio) and POPS/POPS d₃₁ (2∶1 molar ratio) in the presence of C2α domain (40∶1 phospholipid/protein molar ratio). (B) POPC/POPC d₃₁/POPS (40∶40∶20, molar ratio); POPC/POPC d₃₁/POPS (40∶40∶20, molar ratio) in the presence of C2α (molar ratio phospholipid/protein 40∶1); POPC/POPS d₃₁ (80∶20, molar ratio); POPC/POPS d₃₁ (80∶20, molar ratio) in the presence of C2α (molar ratio phospholipid/protein 40∶1). (C) POPC/POPC-d₃₁/POPS/PIP₂ (35∶40∶20∶5, molar ratio); POPC/POPC-d₃₁/POPS/PIP₂ (35∶40∶20∶5, molar ratio); POPC/POPS-d₃₁/PIP₂ (75∶20∶5, molar ratio and POPC/POPS-d₃₁/PIP₂ (75∶20∶5, molar ratio) in the presence of C2α domain (40∶1 phospholipid/protein molar ratio). Error bars indicate the S.E. (n = 3).