Temperature dependent changes of the light scattering measured at 340 nm of the wild type HspB1 (1) and the G34R (2), P39L (3) and E41K (4) mutants.

<p>Protein samples (0.3 mg/ml) in buffer F (20 mM HEPES/NaOH pH 7.5, containing 100 mM NaCl and 2 mM DTT) were heated with a rate of 1°C/min in the range of 20–85°C. The data presented are mean values of four measurements with error bars corresponding to standard deviation.