Characterisation and fluorescence intensity of tryptophan mutant proteins.

a<p>(M⁻¹s⁻¹ (×10⁵).</p>b<p>Melting temperature (T_m in °C) is defined as the inflection point of the thermal unfolding.</p>c<p>Percentage (%) change in fluorescence intensity calculated at 340 nm. Abbreviations are as follow (NC) = no change; (∼) = not determined; (U) = uninterpretable. All experiments are the average of three independent replicates.