Post-exposure treatment of DOG4 RABV-infected mice with TriGAS dramatically reduces the accumulation of RABV N protein mRNA in the brain.

<p>Mice were infected i.n. with 10⁵ FFU of DOG4 RV and 4 hr later either mock treated with PBS or treated with 10⁷ FFU of TriGAS. Ten to 15 mice per group were euthanized for each tissue at each indicated time point, and the number of DOG4 RABV N mRNA copies in A) whole brain, B) olfactory bulb and C) hypothalamus was quantified by qRT-PCR as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087180#s4" target="_blank">Materials and Methods</a>. The results are presented as the mean RABV N mRNA copy numbers (+SE) per 1,000 copies of L13 mRNA. The bars indicate significant differences in copy numbers between mock-treated and PET-treated animals. *p<.05, **p<.01 and ***p<.001, respectively.