Determination of the expression stability (<i>M</i>) of the 10 candidate genes using the geNorm applet.

<p>(A) Left, the least stable gene was excluded stepwise by calculating <i>M</i> values across 50 ovarian tissues. The average expression stability values and the associated order from least to most stable expression are presented on the y-axis and x-axis, respectively. Right, the determination of the optimal number of internal control genes for normalization depending on the pairwise variation (V) analyses. (B–C) Selection of the most suitable reference genes for normalization in serous (B) and mucinous (C) ovarian tissues using the geNorm program.