Gene stability values and the optimal number of selected reference genes by geNorm.

<p>geNorm analysis of diploid (a, b), tetraploid (c, d), hexaploid (e, f), and octoploid (g, h) embryos and the total sample (i, j). A measure of gene expression stability (M value) of 12 candidate reference genes (a, c, e, g, i). Lower M values indicate more stable expression. Determination of the optimal number of reference genes for normalization was conducted (b, d, f, h, j). The cut-off value was 0.15, which means that if the V value was lower than 0.15, then adding an additional reference gene was not required.