Deletion of <i>bcbem1</i> affects the production of conidia and sclerotia.

<p>(<b>A</b>) Δ<i>bcbem1</i> is severely impaired in conidiation. Wild type (WT), Δ<i>bcbem1</i> and the complemented strain Δ<i>bcbem1C</i> were incubated for 14 d on solid complete medium. Then, conidia were collected and quantified. (<b>B</b>) Δ<i>bcbem1</i> produces more sclerotia on different rich media. Sclerotia production was tested on different solid media: synthetic complete medium (CM), potato dextrose agar without (PDA) and PDA with 10% mashed bean leaves (PDAB). Cultures (10 per strain and condition) were incubated for 14 d in constant darkness. Sclerotia formed by wild type B05.10, Δ<i>bcbem1</i> and Δ<i>bcbem1</i>C on CM (upper panel); numbers of sclerotia produced on different media (lower panel).