E7 enhances UBF1-driven transcription on the rDNA gene promoter.

<p>The pHrDNA-IRES-Luc plasmid and the internal control pRLTK were cotransfected into MCF7 cells along with pcDNA3.1(Control) and the indicated expression vectors. After 48 h, both firefly and <i>Renilla</i> luciferase activity were measured. Reporter activity was calculated as the ratio of firefly luciferase activity to reference <i>Renilla</i> luciferase activity, and normalized so that that luciferase activity in control vector transfected cells equaled 1. The data are representative of three independent experiments and are the means of triplicate samples +/− SD (*p<0.05, **p<0.01, ***p<0.001). Expression of p14^ARF and E7 was monitored by western blot (B), and quantitative real time RT-PCR (C), respectively.