siRNA silencing of target host chaperones.

<p>Knockdown efficiencies of Silencer Select siRNA library (Life Technologies) were quantified, using qRT-PCR to measure the transcript levels of the respective target genes. HeLa cells were transfected with siRNA molecules targeting chaperone proteins, or negative control targets (GAPDH, scrambled siRNA), and RNA samples were purified 48 hours post transfection for analysis by qRT-PCR. Analysis was performed for both pre-validated single siRNA constructs and the constructs in the duplicate sets that represent independent siRNAs targeting the same gene and had not been previously validated. siRNA constructs that showed significant down-regulation compared to siRNA control are designated by asterisk (P<0.05). Data are average values from three independent assays.