Validation of mRNA knock-down by siRNAs found to alter HIV infection.

<p>293T cells were transfected with siRNA against the identified genes and two non-targeting scramble siRNAs. 72 h post-transfection, total RNA was harvested and used to make a cDNA library. The presence of the target gene and a housekeeping gene, TBP, was measured using QPCR. Target gene levels were normalized to TBP within in each sample. Values reported are normalized target gene levels compared to values observed in transfections with non-targeting scramble siRNAs.