Expression of FOXM1 target genes in response to cisplatin in A2780 and SKVO3 cells.

<p>A2780 and SKOV3 cells were treated with the indicated concentration of cisplatin for 24-time PCR analysis (A) and western blotting (B). Results are shown from three independent experiments in triplicates. Columns, mean; bars, SD. (C) A2780 and SKOV3 cells were treated with 2 µg/ml and 4 µg/ml cisplatin for 12 h respectively, then were cultured in fresh complete medium for indicated time points (the time when complete medium was added was set as 0 h). Western blot was performed to determine the expression of EXO1 and β-actin. (D) A2780 and SKO3 cells were transfected with FOXM1 siRNA, 48 h later, EXO1 expression was determined by real-time PCR analysis. Results are shown from three independent experiments in triplicates. Columns, mean; bars, SD. (E) 24 h after FOXM1 siRNA transfection, A2780 and SKOV3 cells were treated with 2 µg/ml and 4 µg/ml cisplatin for 12 h respectively, then were cultured in fresh complete medium for indicated time points (the time when complete medium was added was set as 0 h). The arrow (↓) indicates transfection and subsequent cisplatin treatment. Western blot was performed to determine the expression of FOXM1, EXO1 and β-actin.