FOXM1 induces the transcriptional activity of the human EXO1 gene through a consensus FHRE site.

<p>(A) Sequence and position of the consensus FHRE sequence on the EXO1 promoter. (B) ChIP assays were done in A2780 and SKOV3 cells. Chromatin fragments of the cells were immunoprecipitated with anti-FOXM1 antibody and negative control IgG and subjected to PCR. 1% of the total cell lysates were subjected to PCR before immunoprecipitation as inputs. (C) Schematic structure of wild-type (WT) and mutant (Mut) forms of FHRE2-containing promoter reporters. (D) Luciferase activity with or without mutation in EXO1 promoter. A2780 and SKOV3 cells were transfected with wild-type or mutant FRHE2-containing reporter, or co-transfected with FOXM1 siRNA and wild-type reporter (pGL-3-Basic and pGL3-EXO1 were used as negative and positive control, respectively), then treated with cisplatin for 24 h. Afterwards, luciferase activity in cells were measured. Three independent experiments were conducted.