Characterization of M-TiPSCs generated under a defined culture condition.
- Yoshikazu Kishino (511678)
- Tomohisa Seki (511673)
- Jun Fujita (306534)
- Shinsuke Yuasa (67534)
- Shugo Tohyama (511676)
- Akira Kunitomi (511675)
- Ryota Tabei (563269)
- Kazuaki Nakajima (563270)
- Marina Okada (511679)
- Akinori Hirano (563271)
- Hideaki Kanazawa (563272)
- Keiichi Fukuda (67538)
Publication date
May 2014
DOIAbstract
<p>(A): ALP staining in M-TiPSCs. (B): QT-PCR analyses of M-TiPSCs for the ESC marker genes <i>OCT3/4</i>, <i>NANOG</i>, <i>SOX2</i>, <i>KLF4</i>, <i>c-MYC</i>, and <i>TERT1</i>. (C): QT-PCR analyses of M-TiPSCs for the transgenes, <i>OCT3/4</i>, <i>SOX2</i>, <i>KLF4</i>, and <i>c-MYC</i>. (D): Immunofluorescence staining for pluripotency and surface markers (NANOG, OCT3/4, SSEA3, SSEA4, TRA-1–60, and TRA-1–81) in M-TiPSCs1. (E): Heat map analyses of M-TiPSCs, ESCs, and the parental human T cells. (F): Scatter plots comparing the global gene expression profiles of M-TiPSCs with those of T cells and ESCs.</p
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