The <i>Arabidopsis</i> ZIF2 transporter localises at the tonoplast.

<p>(A–C) Confocal laser scanning microscopy images of <i>Arabidopsis</i> wild-type mesophyll protoplasts transiently expressing either YFP alone (A) or the ZIF2.1-YFP (B) or ZIF2.2-YFP (C) fusions under the control of the 35S promoter. Arrowheads point to the YFP signal on the inner side of the chloroplasts and the nucleus. The YFP and chloroplast autofluorescence signals are visualized by green and red coloration, respectively. Scale bars, 10 µm. (D–F) Confocal laser scanning microscopy images of transgenic <i>Arabidopsis</i> root tips expressing either YFP alone (D) or the ZIF2.1-YFP (E) or ZIF2.2-YFP (F) fusions under the control of the 35S promoter. The YFP signal is visualized by green coloration. Scale bars, 50 µm. (G–R) Confocal laser scanning microscopy images of tobacco leaf epidermal cells transiently co-expressing the ZIF2.2-GFP fusion (G, J, M, P) with the plasma membrane marker PIP2A-mCherry (H, K) or the tonoplast marker g-TIP-mCherry (N, Q) under the control of the 35S promoter. Merged images of whole-cell views (I, O) or nucleus close-ups (L, R) are shown. Arrowheads point to transvacuolar strands and asterisks indicate fluorescence signals approaching the nucleus only on the side facing the exterior of the cell. The GFP and mCherry signals are visualized by green and red coloration, respectively. Scale bars, 20 µm.