Subcellular protein localization using TA-based expression vectors.

<p>A&B, Transient expression of 35S promoter lined with GFP-fused AtSOS2 protein in <i>Arabidopsis</i> mesophyll protoplasts(A), or Ubiquitin promoter linked GFP-fused OsICE1 protein in rice mesophyll protoplasts (B) visualized by fluorescence microscopy. Top: mesophyll protoplasts transformed with empty vectors, Bottom: mesophyll protoplasts transformed with the vector with AtSOS2-GFP (A) or OsICE1-GFP (B). C, Overexpression of the AtSOS2-GFP fusion protein in the root tips of transgenic Arabidopsis by the TA-based pGreen -35S-K expression vector. T3-1, T3-9 and T3-15 indicate three individual T2 transgenic lines overexpressing the AtSOS2-GFP fusion protein.