Detection of phosphorylation of p53 in HMECs incubated with exosomes and its abrogation by NAC.

<p>(A) Western blotting for detection of phosphorylation of p53 at serine 15 (pp53 S15) in HMECs incubated with exosomes from MDA-MB-231 cells for up to 3 h. Equal protein concentrations of cellular lysates were analyzed by western blots for pp53 S15 and total levels of p53. β- actin was used as an equal loading control. (B) Western blot analysis for pp53 S15 in cellular lysates of HMECs untreated and those treated with exosomes from 3 different breast cancer cell lines, MDA-MB-231, T47DA18 and MCF7 respectively for 24 h. β- actin was used as an equal loading control. (C) Western blot analysis for pp53 S15 in cellular lysates of HMECs untreated, treated with NAC alone, treated with NAC and incubated with exosomes, in untreated but incubated with exosomes from MDA-MB-231 cells for 3 h. Tubulin was used as an equal loading control.