Southern blot analysis of transgenic pepper plants carrying <i>J1-1</i> gene.

<p><b>A,</b> Schematic diagram of the T-DNA representing restriction enzyme sites and primer sites for i-PCR. LB, T-DNA left border repeat; RB, T-DNA right border repeat; <i>HPT1</i>, hygromycin phosphotransferase I; CaMV35S, CaMV 35S promoter; T_NOS, transcriptional terminator of nopaline synthase (<i>NOS</i>); T_35S, CaMV 35S transcriptional terminator. <b>B,</b> Southern blot analysis. gDNA was digested with <i>Hin</i>dIII, and hybridized with ³²P-labeled <i>HPT1</i> probe (left) or rehybridized with the <i>J1-1</i>gene (right). WT, non-transformed wild-type pepper plant. Arrowheads indicate endogenous J1-1 bands.