Molecular analysis of transgenic tomato plants.

<p>(A) Independently derived transgenic lines were detected via PCR using the primers RNAi-F/RNAi-R and CHSA-F/OCS-R (lanes 1–9: independent <i>Rs-crt</i> transgenic lines). (B) Independent transgenic lines were detected via RT-PCR using the primers RNAi-F/RNAi-R (lanes 1–5: RNA from <i>Rs-crt</i> transgenic lines 1, 2, 3, 4 and 5). M, DNA marker (DL2000); B, blank control without template; V, empty transformation vector plant; W, wild-type plant (negative control); P, positive plasmid control. (C) Southern blot analysis of <i>Nde</i>I-digested gDNA from T0 transgenic plant leaves (lanes v and g: gDNA from empty transformation vector plants and e<i>gfp</i> transgenic plants (controls); lanes 1–5, gDNA from <i>Rs-crt</i> transgenic lines 1, 2, 3, 4 and 5).