Percentage of cells exhibiting inducible respiratory burst for WT HL-60 and R38+ and R38- RA-resistant HL-60 cells.

<p>Oxidative metabolism (respiratory burst) at 72 h after sequential treatment with two inducing agents during the precommitment (24 h) and subsequent lineage-commitment phase (RA/RA, RA/D₃, RA/-, D₃/D₃, D₃/RA, and D₃/-). Respiratory burst activity in RA-resistant HL-60 cells was only marginally rescued by D₃ only when present both in precommitment and commitment stages of differentiation. WT HL-60 cells exhibit significant respiratory burst when RA or D₃ is present during the lineage-commitment phase. Gates to determine percent increase of expression with treatment were set to exclude 95% of the DMSO control population.