<p>YPD-grown cultures of exponential <i>C. parapsilosis</i> wild type (WT), its isogenic mutant (KO), deficient in <i>CpATC1</i> gene, and the reintegrant (RE) strains were adjusted to a cell density of 1.0×10<sup>6</sup> cells/ml and subjected to the following stress challenges for 1h: 5 mM H<sub>2</sub>0<sub>2</sub>, 50 mM H<sub>2</sub>0<sub>2</sub>, 1.2 M NaCl or 42°C. Identical, untreated samples were maintained at 30°C as a control. Results are expressed as mean ± standard deviation of one representative experiment of two performed in triplicate. Student t-test: *P<0.05; **P<0.01 between WT and RE or KO strains.</p
<p>A. In vitro stress resistance analysis of <i>B. melitensis</i> Δhfq mutant strain. 16 M, 16 MΔhfq...
The theoretical benefits of selection of mutant cell lines, for contributing improved germplasm to ...
<p>The survival of vegetative cells from a saturated culture (Sat), a log phase culture (Log), or sp...
<p>The growth cycle at 30°C of wild type (WT), heterozygous (HET) and homozygous (KO) and reintegran...
R<p>Resistant to nourseothricin (Nou<sup>R</sup>); <sup>S</sup>Sensitive to nourseothricin (Nou<sup>...
<p>SSI-1 and JRS4 wild-type strains, and their corresponding mutant strains from overnight cultures ...
<p>Overnight cultures were inoculated into fresh medium and grown to an OD<sub>600</sub> value of ap...
<p>(A) Expression of exogenous protein was induced with 5 μg ml<sup>-1</sup> tetracycline for 24 h a...
<p>The indicated mutant cells were exposed to the following stress conditions: 400 mM 2-DG (90 min);...
<p>The degree of melanization in response to fungal infection was monitored by visual inspection of ...
<p>The indicated mutant strains, selected for having strongly reduced ability to accumulate SGs (<a ...
Each panel shows cell numbers after application of a physical or chemical stress. Cells were incubat...
<p>(a) Phase contrast microscopy images and flow cytometry profiles showing cell cycle phenotypes of...
Cultures of Chlamydomonas were exposed to a range of relatively mild stresses for a period of 24 h. ...
Mutants with high (yellow bars) and low (blue bars, darker blue indicates more severe defects) wound...
<p>A. In vitro stress resistance analysis of <i>B. melitensis</i> Δhfq mutant strain. 16 M, 16 MΔhfq...
The theoretical benefits of selection of mutant cell lines, for contributing improved germplasm to ...
<p>The survival of vegetative cells from a saturated culture (Sat), a log phase culture (Log), or sp...
<p>The growth cycle at 30°C of wild type (WT), heterozygous (HET) and homozygous (KO) and reintegran...
R<p>Resistant to nourseothricin (Nou<sup>R</sup>); <sup>S</sup>Sensitive to nourseothricin (Nou<sup>...
<p>SSI-1 and JRS4 wild-type strains, and their corresponding mutant strains from overnight cultures ...
<p>Overnight cultures were inoculated into fresh medium and grown to an OD<sub>600</sub> value of ap...
<p>(A) Expression of exogenous protein was induced with 5 μg ml<sup>-1</sup> tetracycline for 24 h a...
<p>The indicated mutant cells were exposed to the following stress conditions: 400 mM 2-DG (90 min);...
<p>The degree of melanization in response to fungal infection was monitored by visual inspection of ...
<p>The indicated mutant strains, selected for having strongly reduced ability to accumulate SGs (<a ...
Each panel shows cell numbers after application of a physical or chemical stress. Cells were incubat...
<p>(a) Phase contrast microscopy images and flow cytometry profiles showing cell cycle phenotypes of...
Cultures of Chlamydomonas were exposed to a range of relatively mild stresses for a period of 24 h. ...
Mutants with high (yellow bars) and low (blue bars, darker blue indicates more severe defects) wound...
<p>A. In vitro stress resistance analysis of <i>B. melitensis</i> Δhfq mutant strain. 16 M, 16 MΔhfq...
The theoretical benefits of selection of mutant cell lines, for contributing improved germplasm to ...
<p>The survival of vegetative cells from a saturated culture (Sat), a log phase culture (Log), or sp...