Phenotypic analysis of the <i>C. parapsilosis</i> strains.

<p>The growth cycle at 30°C of wild type (WT), heterozygous (HET) and homozygous (KO) and reintegrant (RE) yeast strains was monitored in YPD (<b>A</b>) or YPtrehalose (<b>B</b>). To check the ability to use different carbon sources, cells from the WT and KO strains were cultured in liquid minimal medium (MM) supplemented with: glucose (<b>C</b>), sucrose (<b>D</b>), trehalose (<b>E</b>) and lactose (<b>F</b>) at 30°C for 6 hours; or in solid MM medium supplemented with glucose (<b>G</b>) or trehalose (<b>H</b>) for 24h. The susceptibility to compounds that affect the cell wall architecture (<b>I</b>) was examined by spotting approximately 10⁵ cells and 10-fold dilutions thereof, on YPD plates containing the indicated compounds at the following concentrations: SDS (0.02%, w/v); Calcofluor White (60 µg/ml); Congo Red (100 µg/ml) and Caffeine (50 mM). The plates were incubated at 30°C for 48 h and photographed. Growth in liquid medium was measured by cell density at OD₆₀₀. Results are expressed as mean ± standard deviation of one representative experiment of two performed in triplicate. Growth in solid medium was monitored by visual inspection of plates performed in duplicate and repeated twice with similar results.