Ly6C^hi CCR2⁺ inflammatory monocytes and Ly6C^int Ly6G⁺ neutrophils are recruited to the blood and spleen in response to <i>T. gondii</i> profilin.

<p>Mice (n = 3–4/group) were stimulated i.v. with 100 ng rPRF (grey) or PBS (black) then blood (A) and spleen (B) cells were collected 4 hours later and analyzed for expression of CD45, CD11b, Ly6C, Ly6G, and CCR2 by flow cytometry. Gating and analysis was conducted on singlet, live, CD45⁺ cells. CD11b⁺ Ly6C^hi monocytes are within the red elliptical gates and were CCR2⁺ Ly6G⁻. Neutrophils are shown in the blue square gates and were CCR2⁻ Ly6G⁺. The data shown are from one experiment, representative of at least 5 independent experiments. One data point from the PBS group was a statistical outlier, as calculated by Grubb's test, and was excluded from analysis. * indicates p<0.05, ** indicates p<0.01, *** indicates p<0.001.