NOD inhibits cell proliferation.

<p>(a) HUVECs were seeded in different cell densities in 96 well plates and the next day treated for 48 hrs with different concentrations of NOD. During the last 16 hrs the cells were pulsed with 0.2 µCi 3[H] thymidine and subsequently harvested. The results of a representative experiment are depicted and expressed as mean 3H-thymidine incorporation in counts per minute (cpm) ± SD of 6 replicate wells for each condition. A total of 6 experiments were performed with essentially similar results. Significance was defined as *<i>P</i><0.05 and **<i>P</i><0.01 compared to untreated cells, <i>ns</i>: not significant. (b) In separate experiments HUVECs were stimulated with 100 µM of NOD or left untreated. One part of the cells was used for cell cycle analysis, for the other part total RNA was isolated and the expression of AUROKA, CCNB1, CCNA2 and CDK1 was assessed by qPCR (c). A total of three experiments were performed. The results in graph (b) are expressed as mean percentage of cells in G0/1-, S- and G2/M- phase. *<i>P</i><0.05, NOD vs. untreated. The results in graph (c) are expressed as normalized mRNA levels relative to the untreated medium control cells. ** <i>P</i><0.01 vs. untreated control.