Inflammatory response in RP105^−/− mice.

<p>Blood from WT and RP105^−/− mice was collected, diluted (1∶25) and incubated for 24 h with LPS (0–75 ng) ex vivo. TNFα (pg/ml) (<b>A</b>) and IL6 (pg/ml) (<b>B</b>) levels in cell-free supernatant were measured by ELISA (n = 5 WT; n = 5 RP105^−/−). Plasma TNFα levels (pg/ml) (<b>C</b>) and SAA1 (µg/ml) (<b>D</b>) in RP105^−/− and WT mice, 1 h after intraperitoneal injection of LPS (1 µg/mouse) (n = 8 WT PBS; n = 9 WT LPS; n = 9 RP105^−/− PBS; n = 10 RP105^−/− LPS). ST2L mRNA (<b>E</b>) and SIGIRR mRNA (<b>F</b>) expression in the adductor muscle group 10 days after induction of HLI, measured by real-time quantitative PCR (n = 6 WT; n = 6 RP105^−/−). (<b>G</b>) Flow cytometry analysis of monocytes and monocyte subtypes (Ly6C^hi and Ly6C^l°) in RP105^−/− and WT mice. Values are presented as total counts in blood (n x10⁶/mL). Fraction of Ly6C^hi (<b>H</b>) and Ly6^l° (<b>I</b>) subtypes of total monocytes in RP105^−/− and WT mice after incubation with LPS or control ex vivo. Activation state of total monocytes (<b>J</b>), Ly6C^hi monocytes (<b>K</b>) and Ly6^l° monocytes (<b>L</b>) in whole blood incubated with LPS or control ex vivo, measured by mean fluorescence intensity (MFI) of CD11b (n = 5 WT PBS; n = 5 WT LPS; n = 5 RP105^−/− PBS; n = 5 RP105^−/− LPS). nd = non-detectable, ctrl = control. All values are presented as the mean ± SEM. *P<0.05, **P<0.01, ***P<0.001.